BBC LABORATORIES
Agricultural & Environmental Microbiological Services

Sampling Procedure

Sample Area

A sample area is defined by plants of the same variety, in the same type of soil, and under the same irrigation and treatment program. It is always beneficial to not sample plants from the edges of the defined sample area, but to go in a few rows to avoid any "edge effects". If the samples are being taken from a vineyard or orchard, it is beneficial to record exactly where the samples were taken for comparison to future samples.

Root Excavation

Small (lower order) roots should be excavated with care to preserve the attachment of the fine terminal feeder roots which will be examined for VAM colonization. A representative composite sample is obtained from the defined sample area (see above) by combining root samples from 6-10 plants within the area being tested. A total of approximately 50 inches (127 cm) of terminal feeder root is necessary for the analysis.

If a drip system is used in vineyards and orchards, these roots will be more easily obtained in the vicinity of the dripper. For other irrigation systems it may be necessary to investigate the best place for excavation.

Sample Collection

The sample roots are to be collected in a labeled, self-sealing plastic bag. Some soil may be included in the bag with the root material. Additional extraction fees may apply for samples in which roots are difficult to locate and/or to extract from the soil.

Upon request, the laboratory will provide a sample kit including: shipping container(s), gel packs (blue ice), sample bags, labels, Chain of Custody forms and instructions for sampling at shipping cost. International shipments will require USDA labels and permits, please inquire before shipping.

Sample Shipment

Enclose the completed Chain of Custody form in a plastic bag. Request VAM Root Colonization under the section for analysis request. Ground shipment is acceptable for VAM samples (next day shipment is not necessary). Ship samples to:

• SAMPLE RECEIVING
• BBC LABORATORIES
• 1217 NORTH STADEM DRIVE
• TEMPE, AZ 85281